Specimen Requirements

Title

Antiphospholipid Antibody Panel

Specimen Requirement

Collect SST/2.0 mL serum, allow to clot for 30 minutes then promptly centrifuge and send.

Alternate collection: Collect 2 light blue tops or 2 red tops, centrifuge within 60 minutes of collection, transfer 1 mL plasma/serum each to 2 screw top plastic vials, indicate plasma/light blue on tubes and send.

Methodology

Immunoassay (IA)

Stability

Room temp: 5 days, Refrigerated: 14 days, Frozen: 30 days

Reference Range

The reference range is determined by the performing laboratory and will show on the report.

Turnaround

9 days

Clinical Use

Test includes:Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM),Phosphatidylserine/Prothrombin (PS/PT) Antibodies (IgG, IgM) & Cardiolipin Antibodies (IgA, IgG, IgM)

The antiphospholipid antibody syndrome (APS) is a clinical-pathologic correlation that includes a clinical event (e.g. arterial or venous thrombosis, pregnancy morbidity) and persistent positive antiphospholipid antibodies (IgM, IgG Cardiolipin or b2GPI antibodies greater than the 99th percentile; or a lupus anticoagulant). International consensus guidelines for APS suggest waiting at least 12 weeks before retesting to confirm antibody persistence. The Systemic Lupus International Collaborating Clinics immunological classification criteria for systemic lupus erythematosus (SLE) include testing for isotype IgA, which has yet to be incorporated into APS criteria. Low level antiphospholipid antibodies may sometimes be detected in the setting of infection, drug therapy or aging.

Test Code

APLP