Culture, Acid Fast Bacilli w/ Smear (AFB/TB)
Collect in a sterile screw top container:
10 mL body fluids or lower respiratory tract specimens (sputum) or
10 mL gastric lavage or
2 mL CSF or
2 grams fresh (unfixed) tissue or
40 mL urine or
1 mL exudate, aspirate, lesion material, ocular specimen or
1 gram or 1 mL stool
Source is required for testing.
Expectorated sputum: Instruct patient to gargle with water and cough deeply. Three separate early morning collections on at least three consecutive days are recommended.
Tissue and biopsy material: Sterile screw top container with small amount of saline, no fixative or preservative.
Gastric lavage fluid: Sterile screw top container. Must be neutralized with sodium bicarbonate within 4 hours of collection.
Continuous Monitoring Broth Culture System and Conventional Culture • Microscopic Exam with Fluorochrome Stain
Room temp: Unacceptable, Refrigerated: 5 days, Frozen: Unacceptable
Negative results available in 42 days; Positive results require additional testing.
Isolation and identification of mycobacteria, including M. tuberculosis.