Specimen Requirements

Title

CULTURE, BLOOD

Specimen Requirement

Obtain 2-3 blood culture sets. Collecting a single set is not recommended. One set will provide insufficient blood volume for the detection of some infections. Obtain blood samples using a syringe or butterfly. Inoculate 1 aerobic bottle (TSB) with 15-20 mL of blood and 1 anaerobic bottle (Thioglycollate broth) with 15-20 mL of blood. Do not vent the vials. Store specimens at room temperature. For pediatric patients, call laboratory for instructions.

Methodology

Conventional culture technique.

Stability

Do not refrigerate of freeze, do not pre-incubate bottles prior to shipment.

Reference Range

By Report.

Turnaround

5-6 days

Clinical Use

Aids in the diagnosis of septicemia, endocarditis, fever of unknown origin, and sepsis.

Test Code

BLCUL