Cytomegalovirus DNA, Quant, Real Time PCR
Collect lavender EDTA/1.0 mL whole blood minimum, send room temp or refrigerated.
Collect EDTA PPT or SST/1.0 mL plasma/serum, centrifuge and transfer plasma/serum to a screw top plastic vial within 2 hours of collection. Indicate sample type on tube and send.
Real-Time Polymerase Chain Reaction (PCR)
Room temp: 48 hours, Refrigerated: 8 days, Frozen: Unacceptable
Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.