Specimen Requirements

Title

Intrinsic Factor Blocking, AB

Specimen Requirement

DRAW BLOOD IN A SST/1.0 mL.  SPIN, SEPARATE SERUM FROM CELLS WITHIN 2 HOURS.

VERY HIGH SERUM LEVELS OF VITAMIN B12 MAY GIVE FALSE POSITIVE RESULTS FOR INTRINSIC FACTOR ANTIBODY. NO SAMPLE SHOULD BE COLLECTED FROM A PATIENT WHO HAS RECEIVED VITAMIN B12 INJECTION WITHIN THE PAST WEEK.

Methodology

Stability

Room Temperature 8 Hours; Refrigerated 48 Hours

Reference Range

By Report*

Turnaround

3-5 days

Clinical Use

Differentiation of pernicious anemia from other megaloblastic anemias. Intrinsic factor blocking autoantibodies are found in approximately 75% of patients with pernicious anemia. High serum concentrations of vitamin B12 (cobalamin) cause false positive results.

Test Code

IFA