Specimen Requirements

Title

LYME DISEASE IgG/IgM ANTIBODY (REFLEX TO IMMUNOBLOT)

Specimen Requirement

1 SST/1.0 mL serum. After 30 minutes of clotting, promptly centrifuge and refrigerate.

Methodology

Stability

Room temp 8hrs; Refrigerated 7 days;Frozen 14 days

Reference Range

By report*

Turnaround

2 days Performed Mon, Wed, Fri

Clinical Use

Whole-cell sonicate (WCS) Borrelia burgdorferi antigens are utilized to measure both IgM and IgG antibody responses to B. burgdorferi in human serum, using an enzyme-immunoassay methodology (EIA). This test is used as a screen in a two-tier test algorithm proposed by the CDC for assessment of exposure to B. burgdorferi during early- and late-stage disease. For determination of early-stage Lyme disease (within the first 4 weeks of onset of erythema migrans), both IgM and IgG antibody detection should be evaluated. For late-stage Lyme disease (after 4 weeks of onset of erythema migrans), only IgG antibody detection should be evaluated. All IgM and IgG EIA positive results should be confirmed using the second tier immunoblot (IB) method.

Test Code

LYMEW