LYME DISEASE IgG/IgM ANTIBODY (REFLEX TO IMMUNOBLOT)
1 SST/1.0 mL serum. After 30 minutes of clotting, promptly centrifuge and refrigerate.
Room temp 8hrs; Refrigerated 7 days;Frozen 14 days
2 days Performed Mon, Wed, Fri
Whole-cell sonicate (WCS) Borrelia burgdorferi antigens are utilized to measure both IgM and IgG antibody responses to B. burgdorferi in human serum, using an enzyme-immunoassay methodology (EIA). This test is used as a screen in a two-tier test algorithm proposed by the CDC for assessment of exposure to B. burgdorferi during early- and late-stage disease. For determination of early-stage Lyme disease (within the first 4 weeks of onset of erythema migrans), both IgM and IgG antibody detection should be evaluated. For late-stage Lyme disease (after 4 weeks of onset of erythema migrans), only IgG antibody detection should be evaluated. All IgM and IgG EIA positive results should be confirmed using the second tier immunoblot (IB) method.