Specimen Requirements

Title

HCV Genotype 3 NS5a Drug Resistance

Specimen Requirement

Preferred specimen: Collect SST or PPT (2.0 mL serum/plasma min), centrifuge and send.

Alternate Collection: Collect lavender EDTA, centrifuge, transfer 2.0 mL plasma to a screw top plastic vial, indicate sample type on tube and send.

Order for patient with genotype 3, if genotype is unknown, order test code HCVGQ for HCV Genotype first.

Methodology

Polymerase Chain Reaction (PCR) • Sequencing

Stability

Room Temperature: 72 hours; Refrigerated: 14 days; Frozen: 42 days

Reference Range

The reference range is determined by the performing laboratory and will show on the report

Turnaround

9-14 days (if HCVGQ is also ordered, turn around will be delayed)

Clinical Use

For detection of mutations in the genotype 3 NS5a gene associated with resistance to NS5a inhibitors (example: daclatasvir, velpatasvir) including the Y93H polymorphism.

MUST HAVE GENOTYPE 3!!!

Test Code

HCGT3