Specimen Requirements
Title
HCV Genotype 3 NS5a Drug Resistance
Specimen Requirement
Preferred specimen: Collect SST or PPT (2.0 mL serum/plasma min), centrifuge and send.
Alternate Collection: Collect lavender EDTA, centrifuge, transfer 2.0 mL plasma to a screw top plastic vial, indicate sample type on tube and send.
Order for patient with genotype 3, if genotype is unknown, order test code HCVGQ for HCV Genotype first.
Methodology
Polymerase Chain Reaction (PCR) • Sequencing
Stability
Room Temperature: 72 hours; Refrigerated: 14 days; Frozen: 42 days
Reference Range
The reference range is determined by the performing laboratory and will show on the report
Turnaround
9-14 days (if HCVGQ is also ordered, turn around will be delayed)
Clinical Use
For detection of mutations in the genotype 3 NS5a gene associated with resistance to NS5a inhibitors (example: daclatasvir, velpatasvir) including the Y93H polymorphism.
MUST HAVE GENOTYPE 3!!!
Test Code
HCGT3